The Abumrad lab directs the Adipocyte Biology and Molecular Nutrition (ABMN) Core designed to stimulate and support leading-edge research in nutrition/obesity. The Core provides expertise in cell metabolism, cell biology and access to an array of specialized equipment.
The ABMN Core:
Assists investigators in conduct of specialized metabolic studies: i) evaluation of adipose tissue morphology (cell size, macrophage infiltration, fibrosis), ii) functional assays of cellular uptake, utilization of substrate (fatty acids, glucose), lipolysis, autophagy, iii) cell/organelle imaging to visualize substrate metabolism, iv) cell respiration/ mitochondrial function.
Provides investigators with access to and training in use of specialized equipment that is too costly for individual investigators.
Provides expert consultation/training services in molecular nutrition to clinical researchers, to young investigators transitioning to independence and to established investigators new to nutrition.
Partners with other Cores at Washington University to optimize research on nutrition and associated metabolic pathology.
ABMN CORE SERVICES AND EQUIPMENT
2 ABI 7500 Fast real-time PCR machines
1 Nikon TE2000u Inverted Microscope
2 Oxygraph 2K respirometry systems for mitochondrial studies
1 Beckman Coulter Multisizer III for adipocyte sizing
1 Luminex 200 muliplex immunoassay system
1 Aurora Scientific 405A transducer for measuring muscle fiber mechanics
1 Bio-Tek Synergy II multi-mode plate reader
1 Nanodrop ND-1000 spectrophotometer
1 Li-Cor Odyssey Near IR protein detection system
1 Qiagen TissueLyser II
1 Beckman liquid scintillation counter
1 Tissue and cell culture facility, equipped with: NuAire laminar flow hoods, NuAire CO2 incubators, Lonza Amaxa Nucleofector II transfection system, Nikon TS100 fluorescent microscope, Nikon TMS-F light microscope, Life Technologies automated cell counter, refrigerators, freezers, water baths and centrifuges designated for cell culture
CORE SERVICES AND ACTIVITIES:
a. Cells (mouse and human adipocyte and muscle lines) and differentiation protocols;
b. Adipose tissue morphology (macrophage infiltration, fibrosis, adipocyte sizing
c. Optimized expression panels of genes related to inflammation, autophagy, metabolism FA/glucose uptake, insulin and mTOR pathways, ECM/fibrosis, ER stress.
d. Optimized protein isolation and assays for mTOR, insulin signal transduction, ER stress and inflammation pathways.
e. Metabolic assays; FA/glucose uptake, oxidation, lipolysis, lipogenesis, etc.
f. Mitochondrial physiology; tissue samples and isolated mitochondria.
g. Cellular imaging to visualize steps in substrate processing.
h. Training on microscopy, cell imaging, metabolic assays, mitochondrial function.
i. Consultation on research strategy and design of nutrition related projects.
j. Access to specialized equipment needed for metabolic studies
For more information on Core services and equipment or to schedule a consultation, please contact Core Manager Terri Pietka at 314-362-8459